Lorentz Center - Seeing enzymes in action from 1 Nov 2010 through 5 Nov 2010
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    Seeing enzymes in action
    from 1 Nov 2010 through 5 Nov 2010

 

Program – Seeing Enzymes in Action

 

All lectures take place in room 201 of the Huygens building, unless mentioned otherwise.

 

· Talks are 35 minutes and 10 minutes discussion

· Poster session: posters can be put up in the corridor of the Lorentz Center for the duration of the workshop for all participants to view

· Abstracts

 

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Monday, November 1, 2010

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09:00 – 10:00       Arrival and registration with coffee

10:00 – 10:15       Opening by manager Lorentz Center (Mieke Schutte) en

                               and Introductory remarks by Scientific Organizers

 

Session 1: Catalytic behavior of enzymes at single-molecule level: What do we know?

 

10:15 – 11:00       Hammes: Enzymes in action: conformation coupled catalysis

11:00 – 11:30       Coffee/tea break

11:30 – 12:15       Rigler: Stochastic Events and Correlated Processes in Single

                               Molecule Catalysis

 

12:15 – 13:15       Lunch break @ Gorlaeus Restaurant

 

13:15 – 14:00       Hofkens: Single molecule enzymatics: from model systems to cells

14:00 – 14:35       Canters: FRET-based optical and electrochemical studies of redox

                               enzymes

14:35 – 15:00       Tabares: Picturing the activity of the Cu-containing Nitrite

                               Reductase at the single molecule level

15:00 – 15:25       Goldsmith: Seeing Enzymes (and Fluorescent Proteins) in Action

                               in Solution

15:25 – 15:45       3-paper discussion

15:45 – 16:15       Coffee/tea break

16:15 – 17:00       Robillard, Chairman - Discussion session

 

17:00 – 19:00       Wine & Cheese party

 

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Tuesday, November 2 , 2010

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Session 2: Cell biology and Single molecule studies in vivo: How do enzymes behave in the living cell?

 

09:00 – 09:45       Schmidt: Cell Signaling Molecule-by-Molecule

09:45 – 10:30       Schόtz: Addressing plasma membrane nanostructures by single

                               molecule techniques

10:30 – 11:00       Coffee/tea break

11:00 – 12:00       Spaink - Discussion session

 

12:00 – 14:00       Lunch break @ Gorlaeus Restaurant

 

14:00 – 15:30       Tabares, chairman Flash presentations of Posters

15:30 – 16:00       Coffee/tea break

16:00 – 17:30       Poster Session

 

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Wednesday, November 3, 2010

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Session 3: Technological innovations/challenges: What are the opportunities and pitfalls?

 

09:00 – 09:45       Singh: Electrochemical Sensing in Nanofluidic Channels: A New

                               Single-Molecule Technique

09:45 – 10:30       Haw Yang: Protein Large-Amplitude Conformational Transitions:

                               Dynamics, Mechanics, and Functional Roles

10:30 – 11:00       Coffee/tea break

11:00 – 12:30       Lab tour & Poster session

 

12:30 – 13:45       Lunch break @ Gorlaeus Restaurant

 

13:45 – 14:30       Kapanides: Conformational dynamics of DNA polymerase

14:30 – 15:15       Enderlein: Molecular sizing by measuring translational and rotational diffusion

15:15 – 15:45       Coffee/tea break

15:45 – 16:30       Van Oijen, Chairman: Discussion

 

16:30 – 17:00       Bus transfer to Zijldijk (conference bus at the side of the building)

17:00 – 21:00       Conference Dinner @ Partyboat Groene Hart Cruises

                               More information click here. To sign up click here.

 

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Thursday, November 4, 2010

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Session 4: Protein engineering of catalytic performance:

can we manipulate (conformational heterogeneity in) enzyme catalysis?

 

09:00 – 09:45       Orrit: Photothermal imaging

09:45 – 10:30       Grunwald: Spectrally resolved tracking of single molecules: mRNA

                                export across the nuclear pore

10:30 – 11:00       Coffee/tea break

11:00 – 11:45       Astier: Nanopore and Nanochannel Synthesis for Molecular

                               Interaction Characterisation at the Single-Molecule Level

11:45 – 12:30       Gilardi: Getting “action” from drug metabolising enzymes: exploiting

                               the anatomy of protein assembly for amperometry

 

12:30 – 14:00       Lunch break @ Gorlaeus Restaurant

 

14:00 – 15:15       Jeuken, Chairman Discussion

15:15 – 15:45       Coffe/tea break

15:45 – 16:30       Hildebrandt: Electric field effects on the protein and electron

                               transfer dynamics of immobilized heme proteins – vibrational

                               spectroscopic approaches

16:30 – 17:15       Chen: Single-Molecule Catalysis: Beyond Enzymes

 

 

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Friday, November 5, 2010

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Session 5: Statistics of and theoretical tools for single-molecule behavior: how can

theorists and experimentalists join forces in understanding enzyme catalysis?

 

09:00 – 09:45       Van Oijen: Single-molecule studies of multi-protein machines

09:45 – 10:30       Xie: Single molecule insights into enzyme catalysis and allostery

10:30 – 11:00       Coffee/tea break

11:00 – 11:45       Cao: Non-equilibrium conformational fluctuations in Enzymes

11:45 – 12:30       Chairman Canters,  Discussion

 

12:30 – 14:00       Lunch break @ Gorlaeus Restaurant

 

14:00 – 15:00       Weiss: Multiscale, Superresolved, Ultrasensitive Optical Molecular

                               Imaging

15:00 – 15:30       Tea and Farewell

 

 

===end of workshop===



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